Anticorps Polyclonal de lapin anti-RBM42

RBM42 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 15407-1-AP

Synonymes

RBM 42, RNA-binding motif protein 42, RNA-binding protein 42

---

Galerie de données de validation

• 

  WB analysis using 15407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15407-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15407-1-AP

  IP result of anti-RBM42 (IP:15407-1-AP, 4ug; Detection:15407-1-AP 1:800) with HepG2 cells lysate 1800 ug.

---

Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IP: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15407-1-AP cible RBM42 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM42 Protéine recombinante Ag7667
• Nom complet: RNA binding motif protein 42
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC004204
• Symbole du gène: RBM42
• Identification du gène (NCBI): 79171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM42, also known as RNA-binding motif protein 42, is a member of the RNA-binding protein family that plays critical roles in post-transcriptional regulation of gene expression. Emerging studies highlight RBM42's involvement in pre-mRNA splicing through its association with the spliceosome complex, where it contributes to the precise removal of introns and assembly of mature mRNA. Beyond splicing, RBM42 has been implicated in cellular stress responses, cell cycle regulation, and apoptosis. Dysregulation of RBM42 is linked to developmental disorders and cancers; for instance, mutations in RBM42 are associated with neurodevelopmental abnormalities, while its overexpression or loss correlates with tumor progression in certain malignancies.

{{ptg:RelatedPrimaryAntibodies}}