Anticorps Polyclonal de lapin anti-RBM8A,Y14

• Phare
• Validé par KD/KO

RBM8A,Y14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14958-1-AP

Synonymes

Binder of OVCA1 1, BOV 1, BOV 1A, BOV 1B, BOV 1C, MDS014, RBM8, RBM8A, RBM8A,Y14, RBM8B, Ribonucleoprotein RBM8A, RNA binding motif protein 8A, RNA binding protein 8A, RNA binding protein Y14, Y14, ZNRP, ZRNP1

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Galerie de données de validation

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of Jurkat using 14958-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 14958-1-AP

  IP Result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules MDA-MB-453s
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral humain, tissu ovarien humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14958-1-AP cible RBM8A,Y14 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM8A,Y14 Protéine recombinante Ag6800
• Nom complet: RNA binding motif protein 8A
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC017088
• Symbole du gène: RBM8A
• Identification du gène (NCBI): 9939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	IF	RNA; An atlas of chromatoid body components.; Authors - Oliver Meikar
human	WB,IHC	Front Oncol; RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.; Authors - Yan Lin ; KD Validated
human	WB	Cell Death Discov; CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.; Authors - Xiaojin Song