Anticorps Polyclonal de lapin anti-RBMS1

RBMS1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11061-2-AP

Synonymes

MSSP 3, MSSP 2, MSSP 1, MSSP, HCC 4

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Galerie de données de validation

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  WB analysis using 11061-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11061-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11061-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11061-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11061-2-AP

  IP result of anti-RBMS1 (IP:11061-2-AP, 4ug; Detection:11061-2-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HepG2 using 11061-2-AP

  Immunofluorescent analysis of HepG2 cells, using RBMS1 antibody 11061-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11061-2-AP cible RBMS1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBMS1 Protéine recombinante Ag1493
• Nom complet: RNA binding motif, single stranded interacting protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC018951
• Symbole du gène: RBMS1
• Identification du gène (NCBI): 5937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Epigenetics; Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma; Authors - Hang Zhai
human	RIP	Mol Cell Biol; Increased ANKRD1 Levels in Early Senescence Mediated by RBMS1-Elicited ANKRD1 mRNA Stabilization; Authors - Chang Hoon Shin

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