Anticorps Polyclonal de lapin anti-RBP3

RBP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 14352-1-AP

Synonymes

D10S64, D10S65, D10S66, IRBP, RBP3, RBPI, Retinol binding protein 3

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Galerie de données de validation

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  WB analysis of mouse eye using 14352-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14352-1-AP (RBP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14352-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14352-1-AP (RBP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 14352-1-AP

  IP result of anti-RBP3 (IP:14352-1-AP, 4ug; Detection:14352-1-AP 1:400) with Y79 cells lysate 2000ug.

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  IHC staining of human eye using 14352-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 14352-1-AP (RBP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human eye using 14352-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 14352-1-AP (RBP3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris, cellules Y79
• Résultats positifs en IP: cellules Y79
• Résultats positifs en IHC: tissu oculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14352-1-AP cible RBP3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBP3 Protéine recombinante Ag5699
• Nom complet: retinol binding protein 3, interstitial
• Masse moléculaire calculée: 135 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC039844
• Symbole du gène: RBP3
• Identification du gène (NCBI): 5949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBP3 (retinoid-binding protein 3), also called interstitial retinoid-binding protein (IRBP), is a large glycoprotein that is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix. It is known to bind retinoids and fatty acids. RBP3 migrates around 136-140 kDa in the western blot analysis. (PMID: 15928609)

Publications

Species	Application	Title
mouse	IP	J Exp Med; Identification of a novel cis-regulatory element essential for immune tolerance.; Authors - Taylor N LaFlam
human	IHC	Acta Biomater; Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.; Authors - Majed Felemban
human	WB	Proc Natl Acad Sci U S A; Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy.; Authors - Irene Tom
mouse	WB	Diabetes; Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-induced Retinal Dysfunction and Neurodegeneration through Rhodopsin.; Authors - Jianglei Chen
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
mouse	WB	Sci Rep; Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation; Authors - Mingzhu Yang

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