Anticorps Polyclonal de lapin anti-RBP4

• Phare
• Validé par KD/KO

RBP4 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 11774-1-AP

Synonymes

Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4

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Galerie de données de validation

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  WB analysis using 11774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11774-1-AP (RBP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11774-1-AP

  WB result of RBP4 antibody (11774-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBP4 transfected HepG2 cells.

• 

  WB analysis of rat liver using 11774-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11774-1-AP (RBP4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse eye using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse eye using 11774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RBP4 antibody (11774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: sang humain, cellules HepG2, plasma humain, tissu hépatique de rat
• Résultats positifs en IHC: tissu oculaire de souris, tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

11774-1-AP cible RBP4 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Oie
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBP4 Protéine recombinante Ag2448
• Nom complet: retinol binding protein 4, plasma
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC020633
• Symbole du gène: RBP4
• Identification du gène (NCBI): 5950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Publications

Species	Application	Title
	WB	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
mouse	WB,IF	Bioact Mater; Bio-inspired chiral self-assemblies promoted neuronal differentiation of retinal progenitor cells through activation of metabolic pathway.; Authors - Na Sun
mouse,human	WB	Theranostics; Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.; Authors - Jinxin Liu
mouse	IF	Int J Mol Sci; Retinoid Regulation of Ocular Surface Innate Inflammation.; Authors - Jehan Alam
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
mouse	IHC	Virulence; Altering retinol binding protein 4 levels in hepatitis C: Inflammation and steatosis matter.; Authors - Ming-Ling Chang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human spinal cord (cervical) tissue In 1/100 dilution gave fade labelling

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Human spinal cord (cervical) tissue

RBP4 Antibody Immunohistochemistry validation (1/100 dilution) in Human spinal cord (cervical) tissue (Cat no:11774-1-AP)