Anticorps Polyclonal de lapin anti-RCC1

RCC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 22142-1-AP

Synonymes

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

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Galerie de données de validation

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  WB analysis of A431 using 22142-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22142-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22142-1-AP

  IP Result of anti-RCC1 (IP:22142-1-AP, 3ug; Detection:22142-1-AP 1:1000) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human testis using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 22142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 22142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 22142-1-AP

  Immunofluorescent analysis of A431 cells using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules NIH/3T3
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu testiculaire humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

22142-1-AP cible RCC1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCC1 Protéine recombinante Ag17569
• Nom complet: regulator of chromosome condensation 1
• Masse moléculaire calculée: 421 aa, 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC010067
• Symbole du gène: RCC1
• Identification du gène (NCBI): 1104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Publications

Species	Application	Title
human	IF	Curr Biol; Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.; Authors - Diana Vargas-Hurtado
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	WB	J Med Chem; Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.; Authors - Brianna D Mackie
human	IHC	Cell Death Dis; Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.; Authors - Lijun Qiao
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	J Cell Mol Med; SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing; Authors - Xi Chen