Anticorps Polyclonal de lapin anti-RCL1

RCL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15330-1-AP

Synonymes

RTC2, RPC2, RNAC, RNA 3'-terminal phosphate cyclase-like protein, HSPC338

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Galerie de données de validation

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  WB analysis of A431 using 15330-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15330-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15330-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15330-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15330-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15330-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 15330-1-AP

  Immunofluorescent analysis of A375 cells using 15330-1-AP (RCL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A375, cellules HepG2, cellules MCF-7
• Résultats positifs en IF/ICC: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

15330-1-AP cible RCL1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCL1 Protéine recombinante Ag7058
• Nom complet: RNA terminal phosphate cyclase-like 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC001025
• Symbole du gène: RCL1
• Identification du gène (NCBI): 10171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	J Mol Cell Biol; Stability and function of RCL1 are dependent on interaction with BMS1; Authors - Yong Wang
human	WB,IF	Cancer Cell Int; Rcl1 suppresses tumor progression of hepatocellular carcinoma: a comprehensive analysis of bioinformatics and in vitro experiments.; Authors - Yu Jiaze

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