Anticorps Recombinant de lapin anti-RCN1

• Phare
• Validé par KD/KO

RCN1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240473E9

N° de cat : 83638-3-RR

Synonymes

Reticulocalbin 1, 240473E9, PIG20, RCAL, RCN

---

Galerie de données de validation

• 

  WB analysis using 83638-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83638-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 83638-3-RR

  WB result of RCN1 antibody (83638-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCN1 transfected HeLa cells.

• 

  IF Staining of U2OS using 83638-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RCN1 antibody (83638-3-RR, Clone: 240473E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 83638-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RCN1 Recombinant antibody (83638-3-RR, Clone:240473E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83638-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83638-3-RR against Human RCN1 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HuH-7, cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83638-3-RR cible RCN1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RCN1 Protéine recombinante Ag13256
• Nom complet: reticulocalbin 1, EF-hand calcium binding domain
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 44-46 kDa
• Numéro d’acquisition GenBank: BC010120
• Symbole du gène: RCN1
• Identification du gène (NCBI): 5954
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).

{{ptg:RelatedPrimaryAntibodies}}