Anticorps Polyclonal de lapin anti-RCN2

• Phare
• Validé par KD/KO

RCN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10193-2-AP

Synonymes

Calcium binding protein ERC 55, E6 binding protein, E6BP, ERC 55, ERC55, RCN2, Reticulocalbin 2, TCBP49

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Galerie de données de validation

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  WB analysis using 10193-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10193-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10193-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10193-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10193-2-AP

  IP Result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug.

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 10193-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 10193-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10193-2-AP cible RCN2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCN2 Protéine recombinante Ag0247
• Nom complet: reticulocalbin 2, EF-hand calcium binding domain
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC004892
• Symbole du gène: RCN2
• Identification du gène (NCBI): 5955
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

Publications

Species	Application	Title
mouse	WB	Nat Med; Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy.; Authors - Norihiko Yokoi
rat	WB	Cell Metab; A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.; Authors - Hui Peng
mouse	WB,IF	Proc Natl Acad Sci U S A; Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation.; Authors - Li Lingjie L ; KD Validated
human	IF	Elife; Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking.; Authors - Pornchai Kaewsapsak
human	IF	Elife; Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.; Authors - Victoria Hung
human	WB	Am J Cancer Res; YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling.; Authors - Chengjie Mei