Anticorps Polyclonal de lapin anti-Recoverin

Recoverin Polyclonal Antibody for WB, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

Avis (1)

N° de cat : 10073-1-AP

Synonymes

RCVRN, Cancer associated retinopathy protein, Cancer-associated retinopathy protein, Protein CAR, RCV1

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Galerie de données de validation

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  WB analysis using 10073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10073-1-AP (Recoverin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat retina using 10073-1-AP

  rat retina tissue were subjected to SDS PAGE followed by western blot with 10073-1-AP (Recoverin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 10073-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10073-1-AP (RCVRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse eye using 10073-1-AP

  IP result of anti-Recoverin (IP:10073-1-AP, 4ug; Detection:10073-1-AP 1:1000) with mouse eye tissue lysate 2680 ug.

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  IF Staining of mouse brain using 10073-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Recoverin antibody (10073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 10073-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Recoverin antibody (10073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris, cellules Y79, tissu oculaire de rat, tissu rétinien de rat
• Résultats positifs en IP: tissu oculaire de souris,
• Résultats positifs en IF-P: tissu oculaire de souris, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Informations sur le produit

10073-1-AP cible Recoverin dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Recoverin Protéine recombinante Ag0119
• Nom complet: recoverin
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC001720
• Symbole du gène: Recoverin
• Identification du gène (NCBI): 5957
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.

Publications

Species	Application	Title
rat	WB	J Neurosci; Differentiation of marrow stromal cells into photoreceptors in the rat eye.; Authors - Kicic Anthony A
mouse	WB	iScience; Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease.; Authors - Jie Zhang
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
human	IF	iScience; One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors.; Authors - Yuki Otsuka
human	IF	Sci Rep; Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue.; Authors - Atsushi Kuwahara
mouse	IF	Sci Rep; Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration.; Authors - Kenichi Makabe

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ROs

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