Anticorps Polyclonal de lapin anti-NELFE

• Phare
• Validé par KD/KO

NELFE Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(19)

Avis (1)

N° de cat : 10705-1-AP

Synonymes

NELF E, NELF-E, RD, RDBP, RNA-binding protein RD

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Galerie de données de validation

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  WB analysis of HEK-293 using 10705-1-AP

  WB result of NELFE antibody (10705-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFE transfected HEK-293 cells.

• 

  WB analysis using 10705-1-AP

  Various lyastes were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 10705-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10705-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10705-1-AP

  IP result of anti-NELFE (IP:10705-1-AP, 4ug; Detection:10705-1-AP 1:6000) with mouse brain tissue lysate 1840 ug.

• 

  IHC staining of human lung cancer using 10705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10705-1-AP (NELFE antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10705-1-AP (NELFE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELFE antibody (10705-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of hela using 10705-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NELFE (10705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug rabbit IgG isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu oculaire de souris, tissu placentaire de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ChIP: See 4 publications below
• RIP: See 1 publications below

Informations sur le produit

10705-1-AP cible NELFE dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NELFE Protéine recombinante Ag1186
• Nom complet: RD RNA binding protein
• Masse moléculaire calculée: 37 kDa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC011600
• Symbole du gène: NELFE
• Identification du gène (NCBI): 7936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

Publications

Species	Application	Title
mouse	IF	Nature; The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.; Authors - Bofeng Liu
mouse,human	WB	Cell; Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.; Authors - Sawarkar Ritwick R
mouse	WB,chIP	Nat Commun; Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.; Authors - Li Yu
mouse	WB	Nat Commun; RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.; Authors - Bogang Wu
mouse	WB	Dev Cell; Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.; Authors - Daniel C L Robinson
mouse	WB,IHC	Proc Natl Acad Sci U S A; Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.; Authors - Adelman Karen K

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

HUAI-CHIN (Verified Customer) (02-14-2019)

This antibody works great in Western. Gives me clean single band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse embryonic fibroblast

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