Anticorps Polyclonal de lapin anti-RDH10

• Phare
• Validé par KD/KO

RDH10 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (2)

N° de cat : 14644-1-AP

Synonymes

RDH10, Retinol dehydrogenase 10, SDR16C4

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Galerie de données de validation

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  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14644-1-AP (RDH10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14644-1-AP

  IP result of anti-RDH10(IP:14644-1-AP, 4ug; Detection:14644-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of mouse kidney using 14644-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14644-1-AP (RDH10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14644-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14644-1-AP (RDH10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

14644-1-AP cible RDH10 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, insecte
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RDH10 Protéine recombinante Ag6237
• Nom complet: retinol dehydrogenase 10 (all-trans)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC067131
• Symbole du gène: RDH10
• Identification du gène (NCBI): 157506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).

Publications

Species	Application	Title
human	IF	Nat Commun; Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue.; Authors - Atsushi Kuwahara
mouse	WB	Proc Natl Acad Sci U S A; Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct.; Authors - Tadaaki Nakajima
mouse		Curr Biol; Multiple Isoforms of Nesprin1 Are Integral Components of Ciliary Rootlets.; Authors - Chloe Potter
mouse	WB	Cell Prolif; Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120; Authors - Jun Jing
mouse	IF	Cell Rep; Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.; Authors - Tony DeFalco
human	IF	Stem Cell Reports; Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.; Authors - Albert Lowe

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Claudio (Verified Customer) (06-29-2020)

Antibody did not show specific staining/signal. Antigen retrieval pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PFA-fixed paraffin-embedded mouse sections

FH

Daniel (Verified Customer) (02-12-2019)

Validated with CRISPR/Cas9 knockout cell lines

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO)

RDH10 Antibody Western Blot, validation (1:1000 dilution) in Embryonic stem cells; wild-type (WT), heterozygote (HE), and homozygote (HO) (Cat no:14644-1-AP)