Anticorps Polyclonal de lapin anti-Radixin

Radixin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13790-1-AP

Synonymes

RDX

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Galerie de données de validation

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  WB analysis using 13790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13790-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13790-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 13790-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13790-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Radixin antibody (13790-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Y79, tissu pancréatique de rat, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du foie humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13790-1-AP cible Radixin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Radixin Protéine recombinante Ag4813
• Nom complet: radixin
• Masse moléculaire calculée: 583 aa, 68 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC047109
• Symbole du gène: RDX
• Identification du gène (NCBI): 5962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

Publications

Species	Application	Title
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines
human	WB	Drug Resist Updat; Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane; Authors - Xin Zhang
mouse	WB	Theriogenology; DDX5 regulates asymmetric division of mouse oocytes by modulating the stability of microfilament-associated protein radixin; Authors - Lina Yu

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