Anticorps Polyclonal de lapin anti-Radixin

Radixin Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13790-1-AP

Synonymes

DFNB24, radixin, RDX

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Galerie de données de validation

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  WB analysis of mouse pancreas using 13790-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13790-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13790-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13790-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13790-1-AP (Radixin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 13790-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Radixin antibody (13790-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, cellules HEK-293, cellules Y79
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du foie humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

13790-1-AP cible Radixin dans les applications de WB, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Radixin Protéine recombinante Ag4813
• Nom complet: radixin
• Masse moléculaire calculée: 583 aa, 68 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC047109
• Symbole du gène: RDX
• Identification du gène (NCBI): 5962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

Publications

Species	Application	Title
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines