Anticorps Polyclonal de lapin anti-REEP5

• Phare
• Validé par KD/KO

REEP5 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (5)

N° de cat : 14643-1-AP

Synonymes

C5orf18, D5S346, DP1, Polyposis locus protein 1, Protein TB2, receptor accessory protein 5, REEP5, TB2

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Galerie de données de validation

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  WB analysis using 14643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14643-1-AP

  WB result of REEP5 antibody (14643-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.

• 

  WB analysis using 14643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14643-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14643-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14643-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14643-1-AP

  IP Result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human colon cancer using 14643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 14643-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (14643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HeLa using 14643-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14643-1-AP (REEP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14643-1-AP

  1X10^6 HeLa cells were stained with .2ug REEP5 antibody (14643-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 18 publications below
• IP: See 4 publications below

Informations sur le produit

14643-1-AP cible REEP5 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: REEP5 Protéine recombinante Ag6229
• Nom complet: receptor accessory protein 5
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC065926
• Symbole du gène: REEP5
• Identification du gène (NCBI): 7905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Publications

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Cell; A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.; Authors - Jin Rui Liang ; KO Validated
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human,mouse	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	IP	Mol Psychiatry; Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.; Authors - M G Sharoar
mouse	WB	Nat Chem Biol; A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.; Authors - Yu-Shiuan Cheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (05-10-2022)

Sharp and strong signal at 21 kDa with clear background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLa cell line

REEP5 Antibody Western Blot validation (1:250 dilution) in HeLa cell line (Cat no:14643-1-AP)

FH

Christine (Verified Customer) (11-10-2021)

Very good detection of endogenous REEP5 protein in human HEK293T and HeLa cell lines. Band at about 20 kDa (expected size for REEP5)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Cell culture of HeLa and HEK293T

FH

Wei (Verified Customer) (09-22-2020)

The antibody gets sharp, single band on western blot.

• Applications: Western Blot
• Cell Tissue Type: Mouse heart

FH

WEI (Verified Customer) (06-29-2020)

The staining pattern is consistent with cardiomyocytes like ER localization.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

FH

H (Verified Customer) (04-05-2020)

The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T and HCT116

REEP5 Antibody Western Blot validation (1:3000 dilution) in HEK293T and HCT116 (Cat no:14643-1-AP)

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