Anticorps Polyclonal de lapin anti-RELB

RELB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 25027-1-AP

Synonymes

I REL, IREL, RELB, Transcription factor RelB

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Galerie de données de validation

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  WB analysis using 25027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25027-1-AP (RELB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 25027-1-AP

  IP Result of anti-RELB (IP:25027-1-AP, 3ug; Detection:25027-1-AP 1:300) with Raji cells lysate 2500ug.

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  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 25027-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RELB antibody (25027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 25027-1-AP

  Immunofluorescent analysis of HeLa cells using 25027-1-AP (RELB antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of Jurkat using 25027-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human RELB (25027-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Ramos, tissu de thymus de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

25027-1-AP cible RELB dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RELB Protéine recombinante Ag13824
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog B
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62-65 kDa
• Numéro d’acquisition GenBank: BC028013
• Symbole du gène: RELB
• Identification du gène (NCBI): 5971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Publications

Species	Application	Title
human	WB, IHC	Nat Genet; Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma.; Authors - Lu Jiang
human	IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
human	WB	Cell Death Dis; Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.; Authors - Xinhua Xiao
human	WB	J Clin Endocrinol Metab; Circulating Myeloid-derived Suppressor Cells Facilitate Invasion of Thyroid Cancer Cells by Repressing miR-486-3p.; Authors - Li Chen
human	FC	Front Oncol; NF-κB fingerprinting reveals heterogeneous NF-κB composition in diffuse large B-cell lymphoma; Authors - Eleanor Jayawant
mouse,human	WB	Antioxidants (Basel); Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway; Authors - Qianqian Wang