Anticorps Recombinant de lapin anti-RELB

RELB Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242336G4

N° de cat : 83305-6-RR

Synonymes

Transcription factor RelB, I-Rel, 242336G4

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Galerie de données de validation

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  WB analysis using 83305-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83305-6-RR (RELB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Raji using 83305-6-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug RELB Recombinant antibody (83305-6-RR, Clone:242336G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83305-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83305-6-RR against Human RELB were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules MDA-MB-231
• Résultats positifs en FC (Intra): cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83305-6-RR cible RELB dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RELB Protéine recombinante Ag13824
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog B
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62-65 kDa
• Numéro d’acquisition GenBank: BC028013
• Symbole du gène: RELB
• Identification du gène (NCBI): 5971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

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