Anticorps Polyclonal de lapin anti-RELN

RELN Polyclonal Antibody for IF, IHC,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 20689-1-AP

Synonymes

PRO1598, reelin, RELN, RL

---

Galerie de données de validation

• 

  IHC staining of human liver cancer using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of mouse brain using 20689-1-AP

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig.

• 

  IF Staining of mouse brain using 20689-1-AP

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig.

---

Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

20689-1-AP cible RELN dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: reelin
• Masse moléculaire calculée: 388 kDa
• Numéro d’acquisition GenBank: NM_005045
• Symbole du gène: RELN
• Identification du gène (NCBI): 5649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RELN belongs to the reelin family. It is an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. It is a secreted glycoprotein which is essential for neuronal migration in the cortex. RELN regulates microtubule function in neurons and neuronal migration. It affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2, RELN induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2).

Publications

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Reyes (Verified Customer) (03-01-2024)

I tried RELN (in red) in human paraffin brain sections and the result was a very good specificity.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

RELN Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20689-1-AP)

FH

Etienne (Verified Customer) (12-19-2022)

Worked very well at 1/50 and 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoid

RELN Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoid (Cat no:20689-1-AP)