Anticorps Polyclonal de lapin anti-REPS2

REPS2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 20699-1-AP

Synonymes

Partner of RalBP1, POB1, RalBP1 interacting protein 2, REPS2

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Galerie de données de validation

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  WB analysis of mouse brain using 20699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 20699-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 20699-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20699-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20699-1-AP

  Immunofluorescent analysis of HepG2 cells, using REPS2 antibody 20699-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules LNCaP, tissu rénal de souris, tissu testiculaire humain
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

20699-1-AP cible REPS2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RALBP1 associated Eps domain containing 2
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa, 55 kDa, 30 kDa
• Numéro d’acquisition GenBank: NM_004726
• Symbole du gène: REPS2
• Identification du gène (NCBI): 9185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REPS2, also named as POB1, is involved in growth factor signaling through its influence on the Ral signaling pathway. The antibody is specific to REPS2.

Publications

Species	Application	Title
human	IF	Cell Signal; REPS2 downregulation facilitates FGF-induced adhesion and migration in human lens epithelial cells through FAK/Cdc42 signaling and contributes to posterior capsule opacification.; Authors - Chan Wen