Anticorps Polyclonal de lapin anti-RFC2

RFC2 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 10410-1-AP

Synonymes

A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, Replication factor C subunit 2, RF C 40 kDa subunit, RFC2, RFC40

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Galerie de données de validation

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  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10410-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10410-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10410-1-AP

  IP result of anti-RFC2 (IP:10410-1-AP, 4ug; Detection:10410-1-AP 1:500) with HeLa cells lysate 3000 ug.

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  IF Staining of HeLa using 10410-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10410-1-AP (RFC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules Raji
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

10410-1-AP cible RFC2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFC2 Protéine recombinante Ag0663
• Nom complet: replication factor C (activator 1) 2, 40kDa
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC002813
• Symbole du gène: RFC2
• Identification du gène (NCBI): 5982
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).

Publications

Species	Application	Title
mouse	WB	Mol Syst Biol; The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.; Authors - Caron Etienne E
mouse	WB	Proc Natl Acad Sci U S A; Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.; Authors - Chiang Po-Min PM
human	WB	Sci Rep; RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.; Authors - Xu Zhao
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lei (Verified Customer) (11-29-2018)

This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2

RFC2 Antibody Western Blot, validation (1:500 dilution) in HepG2 (Cat no:10410-1-AP)