Anticorps Polyclonal de lapin anti-RFC4

RFC4 Polyclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10806-1-AP

Synonymes

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C subunit 4, RF C 37 kDa subunit, RFC37, RFC4

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Galerie de données de validation

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  WB analysis of MCF-7 using 10806-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10806-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 10806-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10806-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10806-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10806-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 10806-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 10806-1-AP

  Immunofluorescent analysis of HepG2 cells, using RFC4 antibody 10806-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of U2OS using 10806-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 10806-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug RFC4 antibody (10806-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules LNCaP, cellules Raji
• Résultats positifs en IF: cellules U2OS, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

10806-1-AP cible RFC4 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFC4 Protéine recombinante Ag1234
• Nom complet: replication factor C (activator 1) 4, 37kDa
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC017452
• Symbole du gène: RFC4
• Identification du gène (NCBI): 5984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.; Authors - Jing Wang
mouse	WB	Cell Rep; Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.; Authors - Stephen Gray
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang