Anticorps Polyclonal de lapin anti-RFK

RFK Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 15813-1-AP

Synonymes

Flavokinase, RFK, riboflavin kinase, RIFK

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Galerie de données de validation

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  WB analysis of HeLa using 15813-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15813-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15813-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15813-1-AP (RFK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15813-1-AP cible RFK dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFK Protéine recombinante Ag8591
• Nom complet: riboflavin kinase
• Masse moléculaire calculée: 162 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC007069
• Symbole du gène: RFK
• Identification du gène (NCBI): 55312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.

Publications

Species	Application	Title
mouse	WB	Transl Psychiatry; Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.; Authors - Michail S Kukharsky
mouse	WB	Eur J Immunol; Riboflavin (vitamin B2 ) deficiency impairs Nox2 priming and defense against Listeria monocytogenes.; Authors - Schramm Michael M
mouse	WB	Adv Sci (Weinh); Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation; Authors - Mengran Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-21-2023)

Works well in mouse tissue for IHC and WB. Standard antigen retrieval procedure and DAB IHC detection were used in the mouse spinal cord (overnight incubation at 4C). The protein primarily neuronal expression, showing weak but clean and clear signal. WB was done with semi-dry transfer and 4% milk in TBST.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse CNS tissue

RFK Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Mouse CNS tissue (Cat no:15813-1-AP)