Anticorps Monoclonal anti-RGS14

RGS14 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C1B2

N° de cat : CL488-67394

Synonymes

Regulator of G-protein signaling 14, 1C1B2

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Galerie de données de validation

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  IF Staining of HeLa using CL488-67394

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RGS14 antibody (CL488-67394, Clone: 1C1B2 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-67394

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rgs14 Monoclonal Antibody (CL488-67394, Clone:1C1B2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67394 cible RGS14 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RGS14 Protéine recombinante Ag9477
• Nom complet: regulator of G-protein signaling 14
• Masse moléculaire calculée: 566 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC014094
• Symbole du gène: RGS14
• Identification du gène (NCBI): 10636
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.

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