Anticorps Monoclonal anti-RHOA

• Phare
• Validé par KD/KO

RHOA Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

1B3D7

Avis (1)

N° de cat : 66733-1-Ig

Synonymes

ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

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Galerie de données de validation

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  WB analysis using 66733-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66733-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66733-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66733-1-Ig cible RHOA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RHOA Protéine recombinante Ag1141
• Nom complet: ras homolog gene family, member A
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC005976
• Symbole du gène: RHOA
• Identification du gène (NCBI): 387
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

Publications

Species	Application	Title
human	IHC	Cell Death Differ; Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserines.; Authors - Liping Yang
human	WB	Clin Sci (Lond); The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.; Authors - Xueying Hu
human	WB	J Biol Chem; SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion; Authors - Yu-Yuan Zhang ; KD Validated
human	WB	Front Mol Biosci; CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.; Authors - Fan Feng
human	WB	J Cancer; GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer.; Authors - Liu Jie
human	WB	Exp Cell Res; RALY may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer.; Authors - Guanchu Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (12-22-2023)

Worked well for WB in Glioma cells at 1:1000 in BSA

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma