Anticorps Polyclonal de lapin anti-RHOB

• Phare
• Validé par KD/KO

RHOB Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(29)

N° de cat : 14326-1-AP

Synonymes

ARH6, ARHB, h6, Rho cDNA clone 6, Rho-related GTP-binding protein RhoB

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Galerie de données de validation

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  WB analysis using 14326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14326-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14326-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14326-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14326-1-AP

  IP result of anti-RHOB (IP:14326-1-AP, 3ug; Detection:14326-1-AP 1:2000) with HeLa cells lysate 2800ug.

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  IP experiment of mouse brain using 14326-1-AP

  IP result of anti-RHOB (IP:14326-1-AP, 4ug; Detection:14326-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

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  IHC staining of human brain using 14326-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14326-1-AP (RHOB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 14326-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14326-1-AP (RHOB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules HeLa, cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en IP: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14326-1-AP cible RHOB dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHOB Protéine recombinante Ag5593
• Nom complet: ras homolog gene family, member B
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC066954
• Symbole du gène: RHOB
• Identification du gène (NCBI): 388
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rho-related GTP-binding protein RhoB (RHOB) is also named ARH6, ARHB and Rho cDNA clone 6 (h6). RhoB was the first member of the Rho family to be implicated in endosomal trafficking. One study showed that RhoB localizes and activates its downstream target, serine/threonine kinase (PRK1), on endosomes, and acts through this signaling pathway to disrupt the trafficking of internalized EGF receptor from endosomes to a prelysosomal compartment (PMID: 29385717). RhoB is known to be part of the immediate early genetic response to epidermal growth factor, transforming growth factor β, Src activation, or genotoxic stress (PMID:9545335, PMID: 10679283). RhoB was shown to have potential implications for EGF signaling by targeting the activated EGF receptors to the lysosome, which represents an "off-switch" for mitogenic signals (PMID: 1050858). RhoB was also demonstrated to exert a negative regulatory influence on TGF-β-induced transcriptional activation (PMID:9545335). The activity of the RhoB promoter was stimulated by genotoxic treatments indicating its role in the cellular response to DNA damage (PMID:9388198).

Publications

Species	Application	Title
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB,IF	Nat Commun; ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.; Authors - Mingdong Liu ; KO Validated
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Mol Cancer Ther; Expression signatures of the lipid-based Akt inhibitors phosphatidylinositol ether lipid analogues in NSCLC cells.; Authors - Zhang Chunyu C ; KD Validated
rat	WB	Sci Rep; MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.; Authors - Yan Zeng
mouse	WB	Invest Ophthalmol Vis Sci; Epigenetic ALYREF/UHRF1/RHOB Axis in Corneal Wound Healing and Implications for Epithelial Tumorigenesis; Authors - Hua Zhang

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