Anticorps Polyclonal de lapin anti-RHPN2

• Phare
• Validé par KD/KO

RHPN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12671-1-AP

Synonymes

Rhophilin-2, Rhophilin 2, RhoBP, p76RBE, GTP-Rho-binding protein 2

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Galerie de données de validation

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  WB analysis of COLO 320 using 12671-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12671-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12671-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HepG2 using 12671-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RHPN2 antibody (12671-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HepG2
• Résultats positifs en IHC: tissu de côlon humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12671-1-AP cible RHPN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHPN2 Protéine recombinante Ag3353
• Nom complet: rhophilin, Rho GTPase binding protein 2
• Masse moléculaire calculée: 686 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC036447
• Symbole du gène: RHPN2
• Identification du gène (NCBI): 85415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.

Publications

Species	Application	Title
		Sci Signal; RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms.; Authors - Junning Yang
human	WB,IF	Cell Cycle; P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.; Authors - Limei Yan ; KD Validated
mouse	WB	J Biochem Mol Toxicol; Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179; Authors - Jian Zhang

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