Anticorps Monoclonal anti-RIC8A

RIC8A Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1E3B11

N° de cat : 66625-1-Ig

Synonymes

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

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Galerie de données de validation

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  WB analysis of HeLa using 66625-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66625-1-Ig (RIC8A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66625-1-Ig

  IP result of anti-RIC8A (IP:66625-1-Ig, 5ug; Detection:66625-1-Ig 1:2000) with HEK-293 cells lysate 4000 ug.

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  IHC staining of mouse brain using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66625-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 66625-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

66625-1-Ig cible RIC8A dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RIC8A Protéine recombinante Ag1593
• Nom complet: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011821
• Symbole du gène: RIC8A
• Identification du gène (NCBI): 60626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Publications

Species	Application	Title
human	WB	J Clin Invest; Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies; Authors - Gonzalo P Solis
human	WB	J Biol Chem; The guanine nucleotide exchange factor Ric-8A regulates the sensitivity of constitutively active Gαq to the inhibitor YM-254890; Authors - Morgan B Dwyer ; KO Validated

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