Anticorps Polyclonal de lapin anti-RILP

• Phare
• Validé par KD/KO

RILP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA, PLA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 13574-1-AP

Synonymes

Rab-interacting lysosomal protein, PP10141, FLJ31193

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Galerie de données de validation

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  WB analysis of HEK-293 using 13574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13574-1-AP (RILP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13574-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13574-1-AP (RILP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13574-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13574-1-AP (RILP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 13574-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13574-1-AP (RILP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13574-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13574-1-AP (RILP Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 13574-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RILP antibody (13574-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13574-1-AP cible RILP dans les applications de WB, IHC, IF/ICC, ELISA, PLA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RILP Protéine recombinante Ag4472
• Nom complet: Rab interacting lysosomal protein
• Masse moléculaire calculée: 401 aa, 41 kDa
• Poids moléculaire observé: 41-45 kDa
• Numéro d’acquisition GenBank: BC031621
• Symbole du gène: RILP
• Identification du gène (NCBI): 83547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy; Authors - Rahul Kumar
human	PLA	J Extracell Vesicles; Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles.; Authors - Xuefeng Fei
mouse		Autophagy; TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes; Authors - Tian Zhang
human	WB	J Exp Clin Cancer Res; Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.; Authors - Minghui Chang
human	WB	J Biol Chem; A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis.; Authors - Mai E Oguchi ; KD Validated
	WB	Cell Death Dis; TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.; Authors - Jia Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (03-22-2021)

There is weak band between 35 and 50 kD. Seems working with WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK lysate

RILP Antibody Western Blot validation (1:500 dilution) in HEK lysate (Cat no:13574-1-AP)

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