Anticorps Polyclonal de lapin anti-RIOK1

• Phare
• Validé par KD/KO

RIOK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 17222-1-AP

Synonymes

AD034, EC:2.7.11.1, RIO kinase 1, RIO1, Serine/threonine-protein kinase RIO1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 17222-1-AP

  WB result of RIOK1 antibody (17222-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIOK1 transfected HeLa cells.

• 

  WB analysis of HeLa using 17222-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17222-1-AP

  IP result of anti-RIOK1 (IP:17222-1-AP, 4ug; Detection:17222-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human placenta using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 17222-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 17222-1-AP (RIOK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17222-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIOK1 antibody (17222-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu d'estomac de rat, tissu cutané de rat, tissu de cancer de l'estomac humain, tissu de cervelet de rat, tissu de cervelet de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17222-1-AP cible RIOK1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RIOK1 Protéine recombinante Ag11057
• Nom complet: RIO kinase 1 (yeast)
• Masse moléculaire calculée: 568 aa, 66 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC006104
• Symbole du gène: RIOK1
• Identification du gène (NCBI): 83732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656)

Publications

Species	Application	Title
human	WB	Biomedicines; RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.; Authors - Min Yu
human	WB	iScience; Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.; Authors - Laura S Bisogno
human	WB	Cancer Res Treat; Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression.; Authors - Zhiqi Huang
human	WB	Mol Cell; RIOK3 mediates the degradation of 40S ribosomes; Authors - Zixuan Huang ; KD Validated
mouse,human	WB,IP,IHC,IF	Nat Cancer; RIOK1 phase separation restricts PTEN translation via stress granules activating tumor growth in hepatocellular carcinoma; Authors - Fanzheng Meng

{{ptg:RelatedPrimaryAntibodies}}