Anticorps Polyclonal de lapin anti-RNASEH1

• Phare
• Validé par KD/KO

RNASEH1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

41

Avis (2)

N° de cat : 15606-1-AP

Synonymes

H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1

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Galerie de données de validation

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  WB analysis using 15606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15606-1-AP

  IP Result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Detection:15606-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 15606-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules U2OS
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IF: See 17 publications below

Informations sur le produit

15606-1-AP cible RNASEH1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNASEH1 Protéine recombinante Ag7990
• Nom complet: ribonuclease H1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002973
• Symbole du gène: RNASEH1
• Identification du gène (NCBI): 246243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Publications

Species	Application	Title
human	WB	Nature; EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.; Authors - Aparna Gorthi
human	WB	Cell; Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.; Authors - Luciano E Marasco
human	WB	Nature; BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.; Authors - Vaibhav Bhatia ; KD Validated
human	WB	Cell Res; Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.; Authors - Junyu Wu
mouse	IF	Nat Cell Biol; Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.; Authors - Flavia Michelini
human	WB	Mol Cell; METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation.; Authors - Canfeng Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (10-05-2023)

Strong signal, very good detection.

• Applications: Western Blot

FH

Huai-Chin (Verified Customer) (06-08-2019)

Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:3000 for Western, 1:500 for IHC, 1:200 for IF-IC
• Cell Tissue Type: Mammary gland/ MCF7