Anticorps Polyclonal de lapin anti-RNASEL

RNASEL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 22577-1-AP

Synonymes

RNase L, RNS4, RNS 4, EC:3.1.26.-, 2-5A-dependent RNase

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Galerie de données de validation

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  WB analysis using 22577-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 22577-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22577-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNASEL antibody (22577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

22577-1-AP cible RNASEL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNASEL Protéine recombinante Ag17409
• Nom complet: ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
• Masse moléculaire calculée: 741 aa, 84 kDa
• Poids moléculaire observé: 84 kDa; 73 kDa
• Numéro d’acquisition GenBank: BC115697
• Symbole du gène: RNASEL
• Identification du gène (NCBI): 6041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes; Authors - Dario F De Jesus
human	wb	Adv Sci (Weinh); The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.; Authors - Qixue Wang
human	WB	Virol Sin; IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway; Authors - Baisong Zheng
mouse,rat	IF	Apoptosis; The function of RNase L and its degradation mechanism in cardiac acute ischemic injury.; Authors - Qiang Ma
		Cardiovasc Diabetol; Human serum RNase-L level is inversely associated with metabolic syndrome and age.; Authors - Yi-Ting Wang
mouse	WB	iScience; Inhibition of cGAS-Mediated Interferon Response Facilitates Transgene Expression.; Authors - Yajuan Fu

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