Anticorps Polyclonal de lapin anti-RNASET2

RNASET2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13753-1-AP

Synonymes

RNase T2, bA514O12.3, EC:4.6.1.19, Ribonuclease 6, Ribonuclease T2

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Galerie de données de validation

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  IHC staining of mouse spleen using 13753-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 13753-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of transfected cells using 13753-1-AP

  IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules transfectées

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

13753-1-AP cible RNASET2 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNASET2 Protéine recombinante Ag4695
• Nom complet: ribonuclease T2
• Masse moléculaire calculée: 256 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC039713
• Symbole du gène: RNase T2
• Identification du gène (NCBI): 8635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

Publications

Species	Application	Title
human		FEBS J; Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.; Authors - Stefan Schorr
human	IHC	Cell Transplant; Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer.; Authors - Mi Tian
mouse		Cell Signal; Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis; Authors - Tao Zhang
human		Arch Insect Biochem Physiol; Exploring the role of RNASET2 in the immune response of black soldier fly larvae; Authors - Sara Caramella
human	IHC	Adv Sci (Weinh); RNASET2 Deficiency Induces Hepatocellular Carcinoma Metastasis through Cholesterol-Triggered MET Activation; Authors - Yanquan Xu
human		Nat Commun; PMID: 30842415; Authors - Juanjuan Ou

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