Anticorps Polyclonal de lapin anti-RNF20

• Phare
• Validé par KD/KO

RNF20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 21625-1-AP

Synonymes

BRE1, BRE1 A, BRE1A, hBRE1, KIAA2779, ring finger protein 20, RNF20

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Galerie de données de validation

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  WB analysis using 21625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21625-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21625-1-AP

  IP Result of anti-RNF20 (IP:21625-1-AP, 3ug; Detection:21625-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IP experiment of HeLa using 21625-1-AP

  IP Result of anti-RNF20 (IP:21625-1-AP, 3ug; Detection:21625-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of mouse kidney using 21625-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21625-1-AP (RNF20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21625-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21625-1-AP (RNF20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21625-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF20 antibody (21625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21625-1-AP

  Immunofluorescent analysis of Hela cells, using RNF20 antibody 21625-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 1 publications below

Informations sur le produit

21625-1-AP cible RNF20 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF20 Protéine recombinante Ag16066
• Nom complet: ring finger protein 20
• Masse moléculaire calculée: 975 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC110585
• Symbole du gène: RNF20
• Identification du gène (NCBI): 56254
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF20, also named as E3 ubiquitin-protein ligase BRE1A, is a 975 amino acid protein, which contains 1 RING-type zinc finger and belongs to the BRE1 family. RNF20 is a component of the RNF20/40 complex and localizes in the nucleus. RNF20 is a component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B. RNF20 is required for transcriptional activation of Hox genes and recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions.

Publications

Species	Application	Title
mouse	WB	Protein Cell; Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis.; Authors - Xiaojuan Liang
mouse	WB	Nucleic Acids Res; H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation.; Authors - Zhiliang Xu ; KO Validated
mouse	WB	Cell Rep; Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization; Authors - Chia-Yeh Lin
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu
mouse	WB	Cell Prolif; Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming.; Authors - Liying Wang
pig	WB	Cell Prolif; RNF20 affects porcine adipocyte differentiation via regulation of mitotic clonal expansion.; Authors - Ying Zhao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

RNF20 Monoclonal antibody giving a very clear sharp bands and good quality staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC and IF
• Cell Tissue Type: Human colon cancer cells and tissue