Anticorps Recombinant de lapin anti-RNF216

RNF216 Recombinant Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240489G2

N° de cat : 83658-2-RR

Synonymes

240489G2, E3 ubiquitin-protein ligase RNF216, EC:2.3.2.27, RING finger protein 216, RING-type E3 ubiquitin transferase RNF216

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Galerie de données de validation

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  WB analysis of SW480 using 83658-2-RR

  SW480 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 83658-2-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of SW480 using 83658-2-RR

  IP result of anti-RNF216 (IP:83658-2-RR, 4ug; Detection:83658-2-RR 1:1000) with SW480 cells lysate 1280 ug.

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  FC experiment of A431 using 83658-2-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RNF216 Recombinant antibody (83658-2-RR, Clone:240489G2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83658-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83658-2-RR against Human RNF216 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules SW480, cellules Caco-2
• Résultats positifs en IP: cellules SW480,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83658-2-RR cible RNF216 dans les applications de WB, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF216 Protéine recombinante Ag0238
• Nom complet: ring finger protein 216
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 57 kDa, 90-99 kDa, 106~130 kDa
• Numéro d’acquisition GenBank: BC000787
• Symbole du gène: RNF216
• Identification du gène (NCBI): 54476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively. Western analysis detected the ~130 kDa band as indicated in literature (PMID: 21270397).

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