Anticorps Polyclonal de lapin anti-ROBO1-Specific

• Phare
• Validé par KD/KO

ROBO1-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 20219-1-AP

Synonymes

Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, ROBO1-Specific, Roundabout homolog 1, SAX3

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Galerie de données de validation

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  WB analysis using 20219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20219-1-AP (ROBO1-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

20219-1-AP cible ROBO1-Specific dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: roundabout, axon guidance receptor, homolog 1 (Drosophila)
• Masse moléculaire calculée: 181 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_002941
• Symbole du gène: ROBO1
• Identification du gène (NCBI): 6091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development. The antibody is specific to ROBO1.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB	Mol Cell; AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.; Authors - Hana Cho ; KD Validated
human	IF	Chin Med J (Engl); Repression of Smad3 by silencing Robo1 attenuates epithelial-mesenchymal transition in tracheobronchial stenosis; Authors - Chunyan He
human	WB	Aging (Albany NY); MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.; Authors - Zongpu Zhang ; KD Validated
human	WB	Angiogenesis; Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.; Authors - Junhui Liu
human	WB	iScience; Activation of EPHA2-ROBO1 Heterodimer by SLIT2 Attenuates Non-canonical Signaling and Proliferation in Squamous Cell Carcinomas.; Authors - Saumya Srivastava ; KD Validated