Anticorps Polyclonal de lapin anti-ROCK1

• Phare
• Validé par KD/KO

ROCK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

78

N° de cat : 21850-1-AP

Synonymes

p160 ROCK 1, P160ROCK, PRO0435, ROCK1

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Galerie de données de validation

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  WB analysis using 21850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21850-1-AP

  WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells.

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  IHC staining of human lymphoma using 21850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 21850-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21850-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (21850-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C6, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Ramos
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 67 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Informations sur le produit

21850-1-AP cible ROCK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROCK1 Protéine recombinante Ag16317
• Nom complet: Rho-associated, coiled-coil containing protein kinase 1
• Masse moléculaire calculée: 1354 aa, 158 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC113114
• Symbole du gène: ROCK1
• Identification du gène (NCBI): 6093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
mouse	IHC	Acta Biomater; Injectable self-assembling peptide nanofiber hydrogel as a bioactive 3D platform to promote chronic wound tissue regeneration.; Authors - Peng Lou
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang
rat	WB	Oxid Med Cell Longev; Tissue Kallikrein Protects Rat Prostate against the Inflammatory Damage in a Chronic Autoimmune Prostatitis Model via Restoring Endothelial Function in a Bradykinin Receptor B2-Dependent Way.; Authors - Mengyang Zhang