Anticorps Monoclonal anti-ROCK1

• Phare
• Validé par KD/KO

ROCK1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3G5E12

Avis (2)

N° de cat : 66782-1-Ig

Synonymes

3G5E12, EC:2.7.11.1, p160 ROCK 1, p160 ROCK-1, P160ROCK

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Galerie de données de validation

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  WB analysis using 66782-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66782-1-Ig (ROCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66782-1-Ig

  WB result of ROCK1 antibody (66782-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

66782-1-Ig cible ROCK1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ROCK1 Protéine recombinante Ag16317
• Nom complet: Rho-associated, coiled-coil containing protein kinase 1
• Masse moléculaire calculée: 1354 aa, 158 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC113114
• Symbole du gène: ROCK1
• Identification du gène (NCBI): 6093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Publications

Species	Application	Title
human	WB	J Cell Biochem; Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer.; Authors - Ranran Dai
mouse	WB	Am J Transl Res; β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.; Authors - Qian Ban
mouse	WB	Food Funct; Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology; Authors - Shuo Yang
human	WB,IHC	Gene; LINC00645 inhibits renal cell carcinoma progression by interacting with HNRNPA2B1 to regulate the ROCK1 mRNA stability; Authors - Hao Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (09-18-2025)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: CRC cells

FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

• Applications: Western Blot
• Primary Antibody Dilution: 1:700
• Cell Tissue Type: renal Cancer cell line (cake-2,caki-2)

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