Anticorps Recombinant de lapin anti-ROCK1

ROCK1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230423C6

N° de cat : 83166-5-RR

Synonymes

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 83166-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83166-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83166-5-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83166-5-RR (ROCK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83166-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (83166-5-RR, Clone: 230423C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 83166-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ROCK1 Recombinant antibody (83166-5-RR, Clone:230423C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83166-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83166-5-RR against Human ROCK1 were performed. The affinity constant is 0.401 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HeLa, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83166-5-RR cible ROCK1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ROCK1 Protéine recombinante Ag16317
• Nom complet: Rho-associated, coiled-coil containing protein kinase 1
• Masse moléculaire calculée: 1354 aa, 158 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC113114
• Symbole du gène: ROCK1
• Identification du gène (NCBI): 6093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

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