Anticorps Polyclonal de lapin anti-ROCK2-Specific(C-term)

• Phare
• Validé par KD/KO

ROCK2-Specific(C-term) Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 20248-1-AP

Synonymes

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

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Galerie de données de validation

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  WB analysis of HeLa using 20248-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20248-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20248-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20248-1-AP

  Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

20248-1-AP cible ROCK2-Specific(C-term) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Rho-associated, coiled-coil containing protein kinase 2
• Masse moléculaire calculée: 161 kDa
• Poids moléculaire observé: 164 kDa
• Numéro d’acquisition GenBank: NM_004850
• Symbole du gène: ROCK2
• Identification du gène (NCBI): 9475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

Publications

Species	Application	Title
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB,IHC	Cell Prolif; The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells.; Authors - Wencan Ke
human	WB,IHC,IF	Exp Neurol; PMID: 27637803; Authors - Jiong Yue
human	WB	Chin J Nat Med; Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation; Authors - Suping Li
rat	IHC	Oxid Med Cell Longev; Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression.; Authors - Yunqiang He
mouse	WB	ASN Neuro; Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.; Authors - Carlos E Pedraza ; KD Validated