Anticorps Polyclonal de lapin anti-ROM1

ROM1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 21984-1-AP

Synonymes

ROM, ROM1, ROSP1, Tetraspanin 23, Tspan 23, TSPAN23

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Galerie de données de validation

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  WB analysis using 21984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21984-1-AP (ROM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21984-1-AP (ROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 21984-1-AP

  Immunofluorescent analysis of HeLa cells using 21984-1-AP (ROM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris, cellules HeLa, tissu oculaire de rat
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

21984-1-AP cible ROM1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROM1 Protéine recombinante Ag17049
• Nom complet: retinal outer segment membrane protein 1
• Masse moléculaire calculée: 351 aa, 37 kDa
• Poids moléculaire observé: 37-45 kDa
• Numéro d’acquisition GenBank: BC008100
• Symbole du gène: ROM1
• Identification du gène (NCBI): 6094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROM1 (also known as TSPAN23) is a transmembrane protein present in photoreceptor outer segment disc membranes. ROM1 belongs to the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. ROM1 is required for rod photoreceptor viability and the regulation of disk morphogenesis (PMID: 10802659). It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks.

Publications

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
human	IF	Elife; Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform.; Authors - Kevin Achberger
	IHC	Cell Mol Life Sci; A look into retinal organoids: methods, analytical techniques, and applications.; Authors - Tess A V Afanasyeva
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
mouse	WB	PLoS One; Mouse models of human ocular disease for translational research.; Authors - Mark P Krebs
mouse	WB, IHC	PLoS One; Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.; Authors - Poppy Datta