Anticorps Polyclonal de lapin anti-RPE65

• Phare
• Validé par KD/KO

RPE65 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (1)

N° de cat : 17939-1-AP

Synonymes

All-trans-retinyl-palmitate hydrolase, EC:3.1.1.64, EC:5.3.3.22, LCA2, Lutein isomerase

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Galerie de données de validation

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  WB analysis of Y79 using 17939-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17939-1-AP (RPE65 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 17939-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17939-1-AP (RPE65 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17939-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17939-1-AP (RPE65 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 17939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17939-1-AP (RPE65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 17939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17939-1-AP (RPE65 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 17939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RPE65 antibody (17939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 17939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RPE65 antibody (17939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 17939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using RPE65 antibody (17939-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HeLa, cellules PC-3
• Résultats positifs en IHC: tissu oculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de souris,
• Résultats positifs en IF-Fro: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 8 publications below

Informations sur le produit

17939-1-AP cible RPE65 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPE65 Protéine recombinante Ag12356
• Nom complet: retinal pigment epithelium-specific protein 65kDa
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65 kDa, 61 kDa
• Numéro d’acquisition GenBank: BC075036
• Symbole du gène: RPE65
• Identification du gène (NCBI): 6121
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

Publications

Species	Application	Title
human	WB	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu
	WB	J Biol Chem; Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina.; Authors - Zhan Gao
mouse	IF	FASEB J; Prph2 disease mutations lead to structural and functional defects in the RPE.; Authors - Lars Tebbe
rat	IF	Invest Ophthalmol Vis Sci; Dasatinib Plus Quercetin Alleviates Choroid Neovascularization by Reducing the Cellular Senescence Burden in the RPE-Choroid; Authors - Yinhao Wang
mouse	WB	Diabetes Res Clin Pract; Thioredoxin upregulation delays diabetes-induced photoreceptor cell degeneration via AMPK-mediated autophagy and exosome secretion.; Authors - Xiang Ren
rat	WB	Am J Pathol; Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.; Authors - Volha V Malechka

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence

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