Anticorps Polyclonal de lapin anti-RPH3A

RPH3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 11396-1-AP

Synonymes

Rabphilin 3A, Rabphilin, KIAA0985, Exophilin-1, Exophilin1

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Galerie de données de validation

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  WB analysis of COLO 320 using 11396-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11396-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 11396-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using RPH3A antibody (11396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

11396-1-AP cible RPH3A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPH3A Protéine recombinante Ag1956
• Nom complet: rabphilin 3A homolog (mouse)
• Masse moléculaire calculée: 690 aa, 76 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC017259
• Symbole du gène: RPH3A
• Identification du gène (NCBI): 22895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.

Publications

Species	Application	Title
human	WB	Mol Cell; Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells; Authors - Tatsuaki Kurosaki
mouse	WB	Front Aging Neurosci; Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses; Authors - Elena Ferrari
mouse	WB	Sci Rep; Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease; Authors - Doan Phuong Quy Nguyen
rat	WB	Br J Pharmacol; Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats; Authors - Lucia Caffino
mouse	WB,IF	Pharmacol Res; Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease; Authors - Elena Ferrari
rat	WB	Elife; Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis; Authors - Tianzhi Li

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