Anticorps Polyclonal de lapin anti-RPL10A

RPL10A Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, PLA, IP, IF, FC, CoIP

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 16681-1-AP

Synonymes

60S ribosomal protein L10a, Csa 19, NEDD 6, NEDD6, ribosomal protein L10a, RPL10A

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Galerie de données de validation

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  WB analysis of HepG2 using 16681-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16681-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16681-1-AP

  IP Result of anti-RPL10A (IP:16681-1-AP, 4ug; Detection:16681-1-AP 1:700) with HeLa cells lysate 1600ug.

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  IF Staining of HepG2 using 16681-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL10A antibody 16681-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu cérébral humain, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 2 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16681-1-AP cible RPL10A dans les applications de WB, PLA, IP, IF, FC, CoIP et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL10A Protéine recombinante Ag10009
• Nom complet: ribosomal protein L10a
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC006791
• Symbole du gène: RPL10A
• Identification du gène (NCBI): 4736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
Xenopus	WB	Neuron; Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.; Authors - Jean-Michel Cioni
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	WB, CoIP	Adv Sci (Weinh); Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer Cells.; Authors - Guangyan Kan
Xenopus	WB,IF	Cell; Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.; Authors - Jean-Michel Cioni

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

this antibody is very good and didn't show non-specific bands in HEK293T cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

RPL10A Antibody Western Blot validation (1:2000 dilution) in (Cat no:16681-1-AP)