Anticorps Polyclonal de lapin anti-RPL14

RPL14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14991-1-AP

Synonymes

60S ribosomal protein L14, CAG ISL 7, CTG B33, hRL14, L14, ribosomal protein L14, RL14, RPL14

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Galerie de données de validation

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  WB analysis using 14991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14991-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14991-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14991-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14991-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14991-1-AP

  IP Result of anti-RPL14 (IP:14991-1-AP, 4ug; Detection:14991-1-AP 1:1000) with mouse liver tissue lysate 6800ug.

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14991-1-AP (RPL14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14991-1-AP (RPL14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14991-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL14 antibody 14991-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14991-1-AP cible RPL14 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL14 Protéine recombinante Ag6976
• Nom complet: ribosomal protein L14
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC000606
• Symbole du gène: RPL14
• Identification du gène (NCBI): 9045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

Publications

Species	Application	Title
mouse	IF	Cell Res; Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.; Authors - Xiaochen Gai
human	WB,IF	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong
human	CoIP,WB	Cell Discov; The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination; Authors - Duo Wu