Anticorps Polyclonal de lapin anti-RPL18A

RPL18A Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14653-1-AP

Synonymes

60S ribosomal protein L18a, ribosomal protein L18a, RPL18A

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Galerie de données de validation

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  WB analysis of HepG2 using 14653-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14653-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14653-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14653-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14653-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 14653-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 14653-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 14653-1-AP

  Immunofluorescent analysis of HepG2 cells using 14653-1-AP (RPL18A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14653-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules PC-3, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

14653-1-AP cible RPL18A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL18A Protéine recombinante Ag6251
• Nom complet: ribosomal protein L18a
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC066319
• Symbole du gène: RPL18A
• Identification du gène (NCBI): 6142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.

Publications

Species	Application	Title
human	WB	Sci Rep; Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.; Authors - Haiping Tang
mouse	WB	Genetics; Quantitative changes in gimap3 and gimap5 expression modify mitochondrial DNA segregation in mice.; Authors - Riikka Jokinen
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
rat	WB	EMBO Rep; Molecular view of ER membrane remodeling by the Sec61/TRAP translocon; Authors - Sudeep Karki