Anticorps Polyclonal de lapin anti-RPL22

• Phare
• Validé par KD/KO

RPL22 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 25002-1-AP

Synonymes

60S ribosomal protein L22, EAP, EBER associated protein, EBER-associated protein, eL22

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Galerie de données de validation

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  WB analysis of A431 using 25002-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25002-1-AP

  WB result of RPL22 antibody (25002-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL22 transfected A431 cells.

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  WB analysis of HepG2 using 25002-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25002-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25002-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 25002-1-AP

  IP result of anti-RPL22 (IP:25002-1-AP, 4ug; Detection:25002-1-AP 1:500) with A431 cells lysate 1920 ug.

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  IF Staining of HeLa using 25002-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPL22 antibody (25002-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

25002-1-AP cible RPL22 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL22 Protéine recombinante Ag21851
• Nom complet: ribosomal protein L22
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-18 kDa
• Numéro d’acquisition GenBank: BC058887
• Symbole du gène: RPL22
• Identification du gène (NCBI): 6146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.; Authors - Martin Gamerdinger
human,mouse	WB,IP,IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	Cell Death Discov; N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness; Authors - Hongliang Dong
human	WB,IF	Carcinogenesis; The Molecular Landscape of Synchronous Colorectal Cancer Reveals Genetic Heterogeneity.; Authors - Xiangfeng Wang
human	WB	Mol Neurobiol; Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation; Authors - Ruicheng Yang
human	IF	Cell Rep; The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress; Authors - Jennifer Jansen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuki (Verified Customer) (01-14-2021)

Cells were grown on sterile coverslips embedded in a 6-well plate. Cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.2% Triton X-100. After blocking with 2% goat serum in PBS, the protein was detected using anti-PRL22 antibodies followed by reaction with Alexa Fluor488- conjugated secondary antibodies.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: colon cells

RPL22 Antibody Immunofluorescence validation (1:200 dilution) in colon cells (Cat no:25002-1-AP)

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