Anticorps Polyclonal de lapin anti-RPL3

• Phare
• Validé par KD/KO

RPL3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 11005-1-AP

Synonymes

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

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Galerie de données de validation

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  WB analysis of HeLa using 11005-1-AP

  WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells..

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  WB analysis using 11005-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11005-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 11005-1-AP

  IP Result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 11005-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11005-1-AP (RPL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 11005-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11005-1-AP (RPL3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11005-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 11005-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules Raji, tissu placentaire humain, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11005-1-AP cible RPL3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL3 Protéine recombinante Ag1436
• Nom complet: ribosomal protein L3
• Masse moléculaire calculée: 46/27 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC012786
• Symbole du gène: RPL3
• Identification du gène (NCBI): 6122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.; Authors - Elizabeth I Vink ; KD Validated
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
human	WB	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
mouse	WB	Aging (Albany NY); RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.; Authors - Chan Chen
human	WB	Nucleic Acid Ther; Knockdown of Muscle-Specific Ribosomal Protein L3-Like Enhances Muscle Function in Healthy and Dystrophic Mice.; Authors - Betty R Kao
human	IHC	Carcinogenesis; DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.; Authors - Xue Zhang