Anticorps Polyclonal de lapin anti-RPL7

RPL7 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14583-1-AP

Synonymes

60S ribosomal protein L7, humL7 1, ribosomal protein L7, RPL7

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 14583-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14583-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14583-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14583-1-AP

  IP Result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human skin cancer using 14583-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin cancer using 14583-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 14583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

14583-1-AP cible RPL7 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL7 Protéine recombinante Ag6104
• Nom complet: ribosomal protein L7
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29-30 kDa
• Numéro d’acquisition GenBank: BC006095
• Symbole du gène: RPL7
• Identification du gène (NCBI): 6129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

Publications

Species	Application	Title
	WB	Nat Cell Biol; METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis.; Authors - Rui Su
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB,RIP	Biochem Biophys Res Commun; CRISPR-based RNA-binding protein mapping in live cells.; Authors - Baiwen Chen
human	WB	Front Endocrinol (Lausanne); Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells; Authors - Manuel Bulfoni
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang
human	WB	Cell; QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism; Authors - Zhicong Zhao