Anticorps Polyclonal de lapin anti-RPS14

RPS14 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 16683-1-AP

Synonymes

40S ribosomal protein S14, EMTB, ribosomal protein S14, RPS14

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Galerie de données de validation

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  WB analysis using 16683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16683-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16683-1-AP (RPS14 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16683-1-AP (RPS14 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 16683-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPS14 antibody 16683-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of U2OS cells using 16683-1-AP

  IF result of 16683-1-AP (anti-RPS14) in U2OS cell by Dr. Nancy Kedersha. (cytosolic small ribosomal and nucleolus; Green).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

16683-1-AP cible RPS14 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris, arabidopsis
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS14 Protéine recombinante Ag10012
• Nom complet: ribosomal protein S14
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC006784
• Symbole du gène: RPS14
• Identification du gène (NCBI): 6208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].

Publications

Species	Application	Title
human	IF	Nucleic Acids Res; Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.; Authors - Fujimura Ken K
human	WB	Acta Neuropathol Commun; Altered ribosomal function and protein synthesis caused by tau.; Authors - Harrison Tudor Evans
Arabidopsis	WB	J Exp Bot; The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis.; Authors - Ilyeong Choi
human	WB	Free Radic Biol Med; Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.; Authors - Reiko Iida
	WB	J Exp Bot; Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.; Authors - Yanyun Shi
zebrafish	IF	Blood Adv; TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling.; Authors - Oscar A Peña