Anticorps Polyclonal de lapin anti-RPS19

RPS19 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 15085-1-AP

Synonymes

40S ribosomal protein S19, DBA, ribosomal protein S19, RPS19

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Galerie de données de validation

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  WB analysis using 15085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15085-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 15085-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15085-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15085-1-AP

  IP result of anti-RPS19 (IP:15085-1-AP, 4ug; Detection:15085-1-AP 1:500) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human colon cancer using 15085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15085-1-AP (RPS19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 15085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15085-1-AP (RPS19 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, cellules HeLa, cellules K-562, tissu de côlon de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15085-1-AP cible RPS19 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS19 Protéine recombinante Ag7164
• Nom complet: ribosomal protein S19
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC000023
• Symbole du gène: RPS19
• Identification du gène (NCBI): 6223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes. The RPS19 protein is a component of the 40S ribosomal subunit [PMID: 17726054]. Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Among these genes, ribosomal protein S19 (RPS19) is mutated most frequently [PMID:21989989]. It also a p53-related ribosomal protein that possibly involves cellular apoptosis through the BAX/p53 pathway [PMID:22272377].

Publications

Species	Application	Title
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
	WB,IF	Development; Msl3 promotes germline stem cell differentiation in female Drosophila.; Authors - Alicia McCarthy
mouse	IHC	J Proteome Res; Human SOD1-G93A specific distribution evidenced in murine brain of a transgenic model for Amyotrophic Lateral Sclerosis by MALDI Imaging Mass Spectrometry.; Authors - Elena Acquadro
human	WB	Sci Rep; RNA G-quadruplex structures control ribosomal protein production; Authors - DhavalVarshney
human	WB	BBA Clin; Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay.; Authors - Joy Armistead
mouse	WB	Adv Sci (Weinh); Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.; Authors - Zhiyuan Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (04-06-2020)

RPS19 is an abundant protein. I could easily detect a clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

RPS19 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:15085-1-AP)

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