Anticorps Polyclonal de lapin anti-RPS26

• Phare
• Validé par KD/KO

RPS26 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, PLA, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 14909-1-AP

Synonymes

40S ribosomal protein S26, ribosomal protein S26, RPS26

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Galerie de données de validation

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  WB analysis of mouse ovary using 14909-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14909-1-AP (RPS26 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14909-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14909-1-AP (RPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 14909-1-AP

  IP Result of anti-RPS26 (IP:14909-1-AP, 4ug; Detection:14909-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 14909-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPS26 antibody 14909-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14909-1-AP cible RPS26 dans les applications de WB, PLA, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS26 Protéine recombinante Ag6706
• Nom complet: ribosomal protein S26
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC002604
• Symbole du gène: RPS26
• Identification du gène (NCBI): 6231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.

Publications

Species	Application	Title
		Nat Commun; Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.; Authors - Yanqin Yu
human	WB	Nat Commun; Genome-wide search for exonic variants affecting translational efficiency.; Authors - Li Quan Q
human	IHC, WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	WB,IP	Oncogene; The ribosomal protein S26 regulates p53 activity in response to DNA damage.; Authors - Cui D D ; KD Validated
human	WB	Int J Mol Sci; Deficiency of the Ribosomal Protein uL5 Leads to Significant Rearrangements of the Transcriptional and Translational Landscapes in Mammalian Cells.; Authors - Elena S Babaylova

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (09-24-2023)

The esophageal cancer cell line is used. The antibody works very stably, and the concentration can be from 1:1000 to 1:2000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500

RPS26 Antibody Western Blot validation (1:1500 dilution) in (Cat no:14909-1-AP)